The Newly Developed Metabolomics Platform Brings New Insights into Enteroaggregative E. coli

November 26 00:36 2021

New York, USA – November 25, 2021 – With years of experience in metabolomics services, Creative Proteomics has developed a microbial metabolome analysis platform dedicated to providing high-quality, personalized microbial metabolomics services and reliable data support to clients worldwide.

Microorganisms have existed on Earth for at least 3.8 billion years and are found in almost all corners of the biosphere. The diversity of microbial metabolic capabilities and metabolic forms is unmatched by any other organism on Earth. There are approximately 20,000 microbial metabolites that have been elucidated. Metabolites can affect not only the tissues in which microbes colonize (e.g., intestinal flora affects gut health), but also the physiological state of the entire host. Metabolomics can be used to fully understand microbial physiological functions, reflect cellular responses to environmental and genetic changes, and detect metabolic markers for determining health or disease.

Escherichia coli (E. coli) is a common rod-shaped bacterium present in the intestinal tract of humans and animals. Most E. coli are beneficial to the organism, and the few that acquire specific virulence factors can cause urinary tract infections, intestinal infections, sepsis, or meningitis. E. coli that can cause diarrhea are known as enteropathogenic E. coli or laxative E. coli.

Enteroaggregative Escherichia coli (EAEC) is a diarrhea-causing pathogen associated with traveler’s diarrhea, foodborne outbreaks, and sporadic diarrhea in industrialized and developing countries. Regulation of EAEC virulence is mediated by AggR and its negative regulator Aar, which controls the expression of at least 210 genes. One-third of these genes are related to metabolism and transport.

To determine the relevance of AggR/Aar-mediated regulation of EAEC metabolic pathways, researchers at the University of Virginia School of Medicine and the Department of Pediatrics at the University of Virginia Children’s Hospital performed untargeted metabolomic analysis of EAEC042 and EAEC042 aar. Creative Proteomics provided the service for this study, using an LC/MS analysis platform (Waters UPLC Class 1 TOF/Q-TOF mass spectrometer) to analyze the bacterial particles. The results revealed differences in the amounts of phosphatidylethanolamine (PE) and lysophosphatidylethanolamine (LysoPE) lipids between these strains, suggesting the involvement of AggR/Aar in the regulation of lipid metabolism.

“Creative Proteomics has deep global resources and a strong reputation for providing contract services specifically for metabolomics. Our dedicated team provides a broader perspective to support data interpretation, giving clients insight into biological questions and advancing science that changes people’s lives,” said Melissa, Marketing Manager at Creative Proteomics.

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About Creative Proteomics

Creative Proteomics is a professional multi-omics provider serving researchers in the pharmaceutical, biotechnology, agricultural and nutrition industries, as well as academia and government agencies. With a continued focus on quality, Creative Proteomics is proud to meet our worldwide clients’ needs.

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